Research

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Publications

Lee, B.; Van Lanen, S. G.; Iwata-Reuyl, D. “Mechanistic studies of QueF, the nitrile oxidoreductase involved in the biosynthesis of queuosine,” under review, Biochemistry.

Roth, A.; Winkler, W. C.; Regulski, E.; Lee, B.; Lim, J.; Jona, I.; Barrick, J. E.; Ritwik, A.; Kim, J.; Welz, R.; Iwata-Reuyl, D.; Breaker, R. R. “A eubacterial riboswitch selective for the queuosine precursor preQ1 contains an unusually small aptamer domain,” Nature Struct. Mol. Biol., 2007, 14, 308-317.

El Yacoubi, B.; Bonnett, S.; Anderson, J.; Swairjo, M. A.; Iwata-Reuyl, D. and de Crécy-Lagard, V. “Discovery of a new prokaryotic type I GTP cyclohydrolase family,” J. Biol. Chem., 2006, 281(49), 37586-37593.

Swairjo, M. A.; Reddy, R. R.; Lee, B.; Van Lanen, S. G.; Brown, S.; de Crécy-Lagard, V.; Iwata-Reuyl, D.; and Schimmel, P. “Crystallization and preliminary X-ray characterization of the nitrile reductase QueF–a queuosine biosynthesis enzyme,” Acta Cryst., 2005, F61, 945-948.

Overbeek, R., et al. “The Subsystems Approach to Genome Annotation and its Use in the Project to Annotate 1,000 Genomes,” Nucleic Acids Res., 2005, 33(17), 5691-5702.

Van Lanen, S. G.; Reader,  J. S.: Swairjo, M. A..; de Crécy-Lagard, V.; Lee, B.; Iwata-Reuyl, D., “From cyclohydrolase to oxido-reductase: Discovery of nitrile reductase activity in a common fold,” Proc. Natl. Acad. Sci. USA, 2005, 102:12, 4264-4269.

Van Lanen, S.; Iwata-Reuyl, D., “Kinetic Mechanism of the tRNA Modifying Enzyme S-Adenosylmethionine:tRNA Ribosyltransferase-isomerase (QueA),” Biochemistry, 2003, 42:18, 5312-5320.

Van Lanen, S.; Daoud Kinzie, S.; Matthieu, S.; Link, T.; Culp, J.; Iwata-Reuyl, D., “tRNA Modification by S-Adenosylmethionine:tRNA Ribosyltransferase-isomerase (QueA): Assay Development and Characterization of the Recombinant Enzyme,” J. Biol. Chem., 2003, 278:12, 10491-10499.

Iwata-Reuyl, D., “The Biosynthesis of the 7-Deazaguanosine Hypermodified Nucleosides of Transfer RNA,” Bioorg. Chem., 2003, 31, 24-43.

Iwata-Reuyl, D.; Math, S. K.; Desai, S. B.; Poulter, C. D. “Bacterial Phytoene Synthase: Molecular Cloning, Over-Expression and Purification of Erwinia herbicola Phytoene Synthase,” Biochemistry, 2003, 42:11, 3359-3365.

Bai, Y.; Fox, D.; Lacy, J.; Van Lanen, S.; and Iwata-Reuyl, D. “Hypermodification of tRNA in Thermophilic Archae: Cloning, Over-Expression, and Characterization of tRNA-Guanine Transglycosylase from Methanococcus jannaschii,” J. Biol. Chem., 2000, 275:37, 28731-28738.

Daoud Kinzie, S.; Thern, B.; Iwata-Reuyl, D. “Mechanistic Studies of the tRNA-Hypermodifying Enzyme QueA: A Chemical Imperative for the Use of AdoMet as a 'Ribosyl' Donor,” Org. Lett. 2000, 2, 1307-1310.

Iwata-Reuyl, D.; Basak, A.; Townsend, C. A. “b-Secondary Deuterium Kinetic Isotope Effects in the Clavaminate Synthase-Catalyzed Oxidative Cyclization of Proclavaminic Acid and in Related Azetidinone Model Reactions,” J. Am. Chem. Soc. 1999, 121, 11356-11368.

Egan, L. A.; Busby, R. W.; Iwata-Reuyl, D.; Townsend, C. A. “The Probable Role of Clavaminic Acid as the Terminal Intermediate in the Common Pathway to Clavulanic Acid and the Antipodal Clavam Metabolites,” J. Am. Chem. Soc. 1997, 119, 2348-2355.

Iwata-Reuyl, D.; Basak, A.; Silverman, L. S.; Engle, C. A.; Townsend, C. A. "Synthesis and Reaction of Potential Alternate Substrates and Mechanism-Based Inhibitors of Clavaminate Synthase," J. Nat. Prod. 1993, 56, 1373-1396.

Iwata-Reuyl, D.; Townsend, C. A. "Common Origin of Clavulanic Acid and Other Clavam Metabolites in Streptomyces," J. Am. Chem. Soc. 1992, 114, 2762-2763.

Salowe, S.M.; Krol, W.J.; Iwata-Reuyl, D.; Townsend, C.A. "Elucidation of the Order of Oxidations and Identification of an Intermediate in the Multi-Step Clavaminate Synthase Reaction," Biochemistry 1991, 30, 2281-2292.